This study aims to investigate the effects of microwaves, ultrasonic and alkaline pretreatments on olive pomace properties and its biomethane potential. Alkaline pretreatment was found to reduce lipid andfiber contents (especially lignin) and to increase soluble matter. The alkali pretreatment at a dose of 8% under 25°C and for 1 day (w/w TS) removed 96% of initial lipids from the solid olive pomace. Unlike NaOH addition, mild microwaves and ultrasonic pretreatments had no impact on lignin. However, in the case of long microwaves pretreatment (450W-10 min), cellulose and lignin contents were reduced by 50% and 26% respectively. Similarly, the combination of ultrasonic and alkali reagent showed a positive effect on fiber degradation and lipid solubilization as
well as a positive impact on methane production. Statistical analysis highlighted the correlation between NaOH dose, solubilization and methane
production. The alkaline pretreatment at ambient temperature appeared the most energetically efficient.
